TY  - JOUR
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Nikolić, Aleksandra
AU  - Medović, Aleksandar
AU  - Mikić, Aleksandar
AU  - Radović, Svetlana
AU  - Đorđević, Vuk
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/934
AB  - The extracts were prepared from the samples of 3,200-year-old charred pea and bitter vetch seeds from the site of Hissar near Leskovac, South Serbia, using two different DNA extraction procedures. We used CTAB method with some modification and obtained low quantity of ancient DNA in comparison with the second method used - commercial available kit. After the extraction, a whole genome amplification using Phi29 DNA polymerase was performed. The amplified DNAs were used for PCR reaction using primers for 26S rDNA gene, which is located on the nuclear genome. The single band corresponding to 26S rDNA fragment from modern relatives was obtained. We conclude that DNA from charred pea and vetch seed can be extracted and used for further archaeobotanical analysis at the molecular level.
AB  - Primenom dve različite procedure ekstrahovana je drevna DNK iz ugljenisanih semena graška i urova starih 3200 godina sa lokaliteta Hisar kod Leskovca. Korišćena je modifikovana CTAB metoda i dobijena je relativno mala količina drevne DNK u poređenju sa drugom primenjenom metodom - komercijalno dostupnim kitom za izolaciju DNK. Nakon ekstrakcije primenjeno je umnožavanje celog genoma pomoću Phi29 DNK polimeraze. Umnožena DNK je korišćena za PCR reakciju primenom prajmera za 26S rDNK gen, koji je lociran u jedarnom genomu. Dobijen je fragment iste veličine kao i PCR fragment 26S rDNK savremenih srodnika graška i urova. Može se zaključiti da je moguće ekstrahovati drevnu DNK iz ugljenisanih semena graška i urova i koristiti je za dalje arheobotaničke analize na molekularnom nivou.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia
T1  - Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji
EP  - 232
IS  - 1
SP  - 227
VL  - 48
DO  - 10.5937/ratpov1101227J
ER  - 

@article{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Nikolić, Aleksandra and Medović, Aleksandar and Mikić, Aleksandar and Radović, Svetlana and Đorđević, Vuk",
year = "2011",
abstract = "The extracts were prepared from the samples of 3,200-year-old charred pea and bitter vetch seeds from the site of Hissar near Leskovac, South Serbia, using two different DNA extraction procedures. We used CTAB method with some modification and obtained low quantity of ancient DNA in comparison with the second method used - commercial available kit. After the extraction, a whole genome amplification using Phi29 DNA polymerase was performed. The amplified DNAs were used for PCR reaction using primers for 26S rDNA gene, which is located on the nuclear genome. The single band corresponding to 26S rDNA fragment from modern relatives was obtained. We conclude that DNA from charred pea and vetch seed can be extracted and used for further archaeobotanical analysis at the molecular level., Primenom dve različite procedure ekstrahovana je drevna DNK iz ugljenisanih semena graška i urova starih 3200 godina sa lokaliteta Hisar kod Leskovca. Korišćena je modifikovana CTAB metoda i dobijena je relativno mala količina drevne DNK u poređenju sa drugom primenjenom metodom - komercijalno dostupnim kitom za izolaciju DNK. Nakon ekstrakcije primenjeno je umnožavanje celog genoma pomoću Phi29 DNK polimeraze. Umnožena DNK je korišćena za PCR reakciju primenom prajmera za 26S rDNK gen, koji je lociran u jedarnom genomu. Dobijen je fragment iste veličine kao i PCR fragment 26S rDNK savremenih srodnika graška i urova. Može se zaključiti da je moguće ekstrahovati drevnu DNK iz ugljenisanih semena graška i urova i koristiti je za dalje arheobotaničke analize na molekularnom nivou.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia, Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji",
pages = "232-227",
number = "1",
volume = "48",
doi = "10.5937/ratpov1101227J"
}

Jovanović, Ž., Stanisavljević, N., Nikolić, A., Medović, A., Mikić, A., Radović, S.,& Đorđević, V.. (2011). Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(1), 227-232.
https://doi.org/10.5937/ratpov1101227J

Jovanović Ž, Stanisavljević N, Nikolić A, Medović A, Mikić A, Radović S, Đorđević V. Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2011;48(1):227-232.
doi:10.5937/ratpov1101227J .

Jovanović, Živko, Stanisavljević, Nemanja, Nikolić, Aleksandra, Medović, Aleksandar, Mikić, Aleksandar, Radović, Svetlana, Đorđević, Vuk, "Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 48, no. 1 (2011):227-232,
https://doi.org/10.5937/ratpov1101227J . .