TY  - CONF
AU  - Ignjatov, Maja
AU  - Milošević, Mirjana
AU  - Petrović, Dragana
AU  - Medić-Pap, Slađana
PY  - 2007
UR  - http://fiver.ifvcns.rs/handle/123456789/2605
AB  - The bacterium Pseudomonas syringae pv.  tomato is seedborne pathogen, causal agent of bacterial speck disease on tomatoes. Frequent appearance of disease was recorded in growing areas in Serbia (Vojvodina province). Symptoms may cause lesions on fruits deep enough to reduce quality of tomatoes. Infected tomato seed is the most significant source of inoculum, and healthy seed is the best measure for disease prevention. Seeds were collected from several major growing areas of tomato in Vojvodina. A study was carried out to determine presence of this pathogen on commercial tomato seeds using serological and molecular methods of identification. Isolation of pathogen was done according to NSHS CDFA Method So 5.1. Chosen isolates were used for further investigation including referent strain of P. s. pv. tomato (KFB 145), Agricultural faculty, Zemun. Bacterium identification was done using serological method (ELISA test) with appropriate antibodies of Pseudomonas syringae pv. tomato NEOGEN Europe Ltd., Scotland, UK. Molecular technique (PCR) was also used for detection of bacterium (coronatine producing strains) in tomato seeds.
PB  - Leuven : International Society for Horticultural Science
C3  - Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007
T1  - Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds
EP  - 135
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2605
ER  - 

@conference{
author = "Ignjatov, Maja and Milošević, Mirjana and Petrović, Dragana and Medić-Pap, Slađana",
year = "2007",
abstract = "The bacterium Pseudomonas syringae pv.  tomato is seedborne pathogen, causal agent of bacterial speck disease on tomatoes. Frequent appearance of disease was recorded in growing areas in Serbia (Vojvodina province). Symptoms may cause lesions on fruits deep enough to reduce quality of tomatoes. Infected tomato seed is the most significant source of inoculum, and healthy seed is the best measure for disease prevention. Seeds were collected from several major growing areas of tomato in Vojvodina. A study was carried out to determine presence of this pathogen on commercial tomato seeds using serological and molecular methods of identification. Isolation of pathogen was done according to NSHS CDFA Method So 5.1. Chosen isolates were used for further investigation including referent strain of P. s. pv. tomato (KFB 145), Agricultural faculty, Zemun. Bacterium identification was done using serological method (ELISA test) with appropriate antibodies of Pseudomonas syringae pv. tomato NEOGEN Europe Ltd., Scotland, UK. Molecular technique (PCR) was also used for detection of bacterium (coronatine producing strains) in tomato seeds.",
publisher = "Leuven : International Society for Horticultural Science",
journal = "Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007",
title = "Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2605"
}

Ignjatov, M., Milošević, M., Petrović, D.,& Medić-Pap, S.. (2007). Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds. in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007
Leuven : International Society for Horticultural Science., 135-135.
https://hdl.handle.net/21.15107/rcub_fiver_2605

Ignjatov M, Milošević M, Petrović D, Medić-Pap S. Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds. in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007. 2007;:135-135.
https://hdl.handle.net/21.15107/rcub_fiver_2605 .

Ignjatov, Maja, Milošević, Mirjana, Petrović, Dragana, Medić-Pap, Slađana, "Molecular and serological identification of Pseudomonas syringae pv. tomato in commercial tomato seeds" in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007 (2007):135-135,
https://hdl.handle.net/21.15107/rcub_fiver_2605 .

TY  - CONF
AU  - Petrović, Dragana
AU  - Milošević, Mirjana
AU  - Ignjatov, Maja
AU  - Medić-Pap, Slađana
PY  - 2007
UR  - http://fiver.ifvcns.rs/handle/123456789/2604
AB  - Tomato (Lycopersicon esculentum) is a plant species parasitized by a great number of microorganisms. Viruses play a significant role regarding the way and speed of transmission, as well as systemic nature of the infection. The aim of this investigation was to determine the presence of two the most significant viruses on tomato Tomato mosaic virus {ToMV) and Tomato spotted wilt virus (TSWV). ToMV is widespread on all tomato growing areas. ToMV is a very stable and can persist in dry contaminated soil, in infected tomato debris, on or in the seed coat. The Tomato spotted wilt virus also has a wide host range and can affect a number of ornamental plants as well as tomato. Early symptoms of spotted wilt on tomato are difficult to diagnose which is very important for nursery plants production. ELISA test is one of the rapid diagnostics techniques for detection of plant viruses.
Samples of tomato plants with symptoms were collected from different localities in Vojvodina. Plant samples were tested for the presence of viruses by DAS ELISA test, using specific antibodies purchased from LOEWE Biochemica GmbH, including positive and negative control for both viruses individually.
PB  - Leuven : International Society for Horticultural Science
C3  - Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007
T1  - Detection of viruses transmitted by tomato seeds using serological methods
EP  - 136
SP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2604
ER  - 

@conference{
author = "Petrović, Dragana and Milošević, Mirjana and Ignjatov, Maja and Medić-Pap, Slađana",
year = "2007",
abstract = "Tomato (Lycopersicon esculentum) is a plant species parasitized by a great number of microorganisms. Viruses play a significant role regarding the way and speed of transmission, as well as systemic nature of the infection. The aim of this investigation was to determine the presence of two the most significant viruses on tomato Tomato mosaic virus {ToMV) and Tomato spotted wilt virus (TSWV). ToMV is widespread on all tomato growing areas. ToMV is a very stable and can persist in dry contaminated soil, in infected tomato debris, on or in the seed coat. The Tomato spotted wilt virus also has a wide host range and can affect a number of ornamental plants as well as tomato. Early symptoms of spotted wilt on tomato are difficult to diagnose which is very important for nursery plants production. ELISA test is one of the rapid diagnostics techniques for detection of plant viruses.
Samples of tomato plants with symptoms were collected from different localities in Vojvodina. Plant samples were tested for the presence of viruses by DAS ELISA test, using specific antibodies purchased from LOEWE Biochemica GmbH, including positive and negative control for both viruses individually.",
publisher = "Leuven : International Society for Horticultural Science",
journal = "Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007",
title = "Detection of viruses transmitted by tomato seeds using serological methods",
pages = "136-136",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2604"
}

Petrović, D., Milošević, M., Ignjatov, M.,& Medić-Pap, S.. (2007). Detection of viruses transmitted by tomato seeds using serological methods. in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007
Leuven : International Society for Horticultural Science., 136-136.
https://hdl.handle.net/21.15107/rcub_fiver_2604

Petrović D, Milošević M, Ignjatov M, Medić-Pap S. Detection of viruses transmitted by tomato seeds using serological methods. in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007. 2007;:136-136.
https://hdl.handle.net/21.15107/rcub_fiver_2604 .

Petrović, Dragana, Milošević, Mirjana, Ignjatov, Maja, Medić-Pap, Slađana, "Detection of viruses transmitted by tomato seeds using serological methods" in Program and Abstract Book, 2nd International Symposium on Tomato Diseases, Kusadasi, Turkey, 8-12 October 2007 (2007):136-136,
https://hdl.handle.net/21.15107/rcub_fiver_2604 .